OPTIMIZATION OF ITRAQ LABELLING COUPLED TO OFFGEL FRACTIONATION AS A PROTEOMIC WORKFLOW TO THE ANALYSIS OF MICROSOMAL PROTEINS OF MEDICAGO TRUNCATULA ROOTS

Abstract Background Shotgun proteomics represents an attractive technical framework for the study of membrane proteins Grains that are generally difficult to resolve using two-dimensional gel electrophoresis.The use of iTRAQ, a set of amine-specific isobaric tags, is currently the labelling method of choice allowing multiplexing of up to eight samp

Abstract Background Despite scientific evidence that climate change has profound and far reaching implications for public health, translating this knowledge in a manner that supports citizen engagement, applied decision-making, and behavioural change can be challenging.This is especially true for complex vector-borne zoonotic diseases such as Lyme

This study examined individual difference correlates of belief in a narrative about the discovery of giant skeletal remains that contravenes mainstream scientific explanations.A total of 364 participants from Central Europe completed a survey that asked them to rate their agreement with a short excerpt describing the giant skeleton myth.Participant

Since the harmful consequences of the online publication of fake news have emerged clearly, many research groups worldwide have started to work on the design and creation of systems able to detect Collections fake news and entities that share it consciously.Therefore, manifold automatic, manual, and hybrid solutions have been proposed by industry a

The article is considering Hydraulic Air Stick the problem of increasing the performance and accuracy of video face identification.We examine the selection of the several best video frames using various techniques for assessing the quality of images.In contrast to traditional methods with estimation of image brightness/contrast, we propose to utili